ABSTRACT
Nevus of Ota is an uncommon dermal melanosis in the Indian subcontinent and is rarely associated with capillary malformations, classified as type II phakomatosis pigmentovascularis (PPV). It usually presents unilaterally as mottled, slate blue, or dark bro wn macules; bilateral presentation is only seen in a few cases. A 20 - year female presented to Dermatology OPD of a tertiary hospital with bilateral nevus of Ota involving the cheeks, temples, nose, forehead, and sclera of the eyes for one year. She also ha d a port wine stain on the right arm, forearm, back, bittock, and thigh since birth without any other systemic involvement. We treated nevus of Ota with six sessions of 1064 nm picosecond laser every month at 0.8 J/cm 2 with significant improvement in the appearance of the lesions and no adverse effects. This case highlights the importance of thoroughly examining pigmented lesions for additional cutaneous findings and the potential use of a 1064 nm picosecond laser to tr eat such lesions.
ABSTRACT
Background: To evaluate the antimicrobial effect of non-thermal atmospheric plasma on mutans biofilms. Materials: A 100 µl culture of Streptococcus mutans poured into two 120 well flat base microtiter plates, allowed to adhere and incubated overnight using brain heart infusion (BHI). These 120 wells were grouped into four groups, group A-helium treated, group B-plasma treated, group C- chlorohexidine treated and group D-control group (untreated group) with a group size of 30 wells per group. Triphenyl tetrazolium chloride (TTC) essay & Colony Forming Units (CFU) counting was done to check the viability & survival of streptococcus mutans biofilms. Results: The results showed that there was a significant difference in the mean optical density (OD) among the four groups (p<0.001). Post-hoc analysis revealed that untreated samples and Helium had significantly higher mean OD than plasma and chlorhexidine groups. A similar result was also seen using the survival essay by CFU counting. Conclusion: Plasma utilizes the air from the atmosphere, thus rendering plasma a better reach in the oral cavity. This property of plasma can be efficiently used to neutralize Streptococcus mutans biofilms in the oral cavity.
ABSTRACT
Background: Acne vulgaris affects about 85% of adolescents, often extending into adulthood. Though considered to be merely a cosmetic problem, it is associated with considerable psychological impairment, such patients are prone to low self-esteem, low self-confidence and social dysfunction which may lead to anxiety, depression, obsessive compulsiveness and sometimes suicidal ideation. AIM: This study was aimed to assess the impact of acne and its sequelae on the quality of life. Methods: The current cross sectional study was conducted in Patients diagnosed as acne vulgaris attending OPD of HIMSR in department of dermatology for a period of 6 months. Patients aged 15 years and above were included in our study. A detailed history was taken after obtaining consent from all the participants of study. Dermatology life quality index (DLQI) was administered on patients to determine the impact of acne vulgaris on their quality of life (QOL). Results: This study included 200 patients with females being more in number 130 (65%) as compared to male patients which were 70 in number (35%). The mean age was 20.49 with majority of patients being in 15-20 years of age group. Mean DLQI score was 8.22, statistically influenced by the age of the patient, duration and grade of acne, acne scar, and post acne hyperpigmentation. Conclusions: This study showed significant impairment of quality of life in acne patients. Counseling along with early treatment of acne vulgaris both are important as to reduce disease-related psychosocial sequelae and enhancing the efficacy of treatment.
ABSTRACT
Background: Melasma a common skin pigmentary disorder poses a great challenge to clinicians due to unsatisfactory results and high recurrence rate. Many treatment modalities have been tried by clinicians without significant improvement in the lesion. Methods: This cross sectional study was done on 90 patients including both male and female and were diagnosed with moderate to severe melasma. TA 250 mg (thyrodin) bid for six months was prescribed along with topical sunscreen. Digital photography was performed at the first visit and at subsequent visits. The effects of treatment were evaluated by two dermatologists independently. Results were assed clinically and photographically. Result: 90 patients with moderate to severe melasma were enrolled in the study. The average age was 36 years. 44patients (48.8%) had good improvement, 25 patients (27.7%) had excellent improvement and 17 patients (18.8%) had fair improvement and 4 patients (4.4%) had no improvement. Three patients complained about gastric upset. None of the patients had serious systemic side effects, only few had oligomenorrhoea, palpitation. Patient’s satisfaction was similarly noted. Conclusion: oral administration of TA is effective and safe treatment for melasma.
ABSTRACT
Background: Vitiligo is defined as an acquired melanocytopenia of obscure aetiology and is characterized by circumscribed hypomelanosis and depigmentation of skin and hair which is often progressive. The aim of present study is to compare between 3 surgical modalities i.e. miniature punch grafting, thin split-skin thickness grafting and suction blister grafting and to experiment a novel method according to the site, size and location of the lesion and to study the extent of re-pigmentation after doing these procedures and comparison of the results in patients of stable vitiligo. We also aim to assess the complications and disadvantages of different surgical techniques. Methods: The study was conducted on total 60 vitiligo patients, who were divided into 3 groups of 20 each, in the age group of 15-60 years, attending Dermatology Department of Katihar Medical College in the span of 2 yearsResults. Results are comparable overall but vary considerably according to site of lesions. Punch grafting is very good for mobile areas like elbow, ankle and other joints; Thin – thiersch’s skin grafting gives better results for flat areas like trunk, thigh, arms and face while Suction blister grafting gives satisfactory results for lips vitiligo and also over small, oval lesions over flat sites. Conclusions: Thus, it can be finally concluded from this study that the surgical modality for treating a case of vitiligo cannot be generalized. Every patient should be evaluated individually according to anatomical site involved, size and shape of lesion, time required to achieve pigmentation, infrastructure available and patient’s preferences.
ABSTRACT
Alopecia areata (AA) is a disease marked by extreme variability in hair loss, not only at the time of initial onset of hair loss but in the duration, extent and pattern of hair loss during any given episode. This variable and unpredictable nature of spontaneous re-growth and lack of a uniform response to various therapies has made clinical trials in alopecia areata difficult to plan and implement. It is a type of alopecia that affects males and females equally. It occurs in both children and adults. The peak age of occurrence is 20 to 50 years .The most common clinical presentation is asymptomatic shedding of telogen hairs followed by patchy non scarring hair loss in association with nail pitting, Beau’s line and nail dystrophy. The disease may progress from this limited presentation to total loss of all scalp hairs (Alopecia totalis) or all body hair (alopecia universalis) with significant onychodystrophy. Mostly it is characterised by reversible hair loss involving the scalp although others areas of head including eyelashes, eyebrows and beard may also be affected. Although, it is a mostly cosmetic problem but it often has devastating effects on quality of life and self-esteem. The paper aims at providing an overview of Alopecia areata.
ABSTRACT
Detection of virulence gene is a key component in determining the pathogenicity of any isolates because these genes act multi-functionally and multi-factorially. Gyrase specific gene primer in combination of PCR technology allows precise detection of DNA gyrase subunit B2 gene (gyrB2) from different virulent microorganisms. In the present study, forward and reverse primers with a length of 20bp for both were used for detection of gyrB2 genes in clinical isolates of Pseudomonas sp. collected from patients suffering from urinary tract infection (UTI). A total of 12 isolates of Pseudomonas sp. viz., Ps1, Ps2, Ps3, Ps4, Ps5, Ps6, Ps7, Ps8, Ps9, Ps10, Ps11 and Ps12 were used in present study in which gyrB2 gene amplified in all 12 isolates and gave the expected 1130bp PCR product after visualization under gel documentation system in 1.2% agarose gel. This PCR was outstanding in the detection of gyrB2 gene in urinary tract infected patients caused by Pseudomonas sp. species.
ABSTRACT
Detection of virulence gene is a key component in determining the pathogenicity of any isolates because these genes act multi-functionally and multi-factorially. Gyrase specific gene primer in combination of PCR technology allows precise detection of DNA gyrase subunit B2 gene (gyrB2) from different virulent microorganisms. In the present study, forward and reverse primers with a length of 20bp for both were used for detection of gyrB2 genes in clinical isolates of Pseudomonas sp. collected from patients suffering from urinary tract infection (UTI). A total of 12 isolates of Pseudomonas sp. viz., Ps1, Ps2, Ps3, Ps4, Ps5, Ps6, Ps7, Ps8, Ps9, Ps10, Ps11 and Ps12 were used in present study in which gyrB2 gene amplified in all 12 isolates and gave the expected 1130bp PCR product after visualization under gel documentation system in 1.2% agarose gel. This PCR was outstanding in the detection of gyrB2 gene in urinary tract infected patients caused by Pseudomonas sp. species.